Active Recombinant Mouse Hhip

• Cat.No.: Hhip-271M
• Product Overview: Recombinant Mouse Hhip (Accession # AAD31172) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Predicted N Terminal: Lys24 & Asp52
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit Sonic Hedgehog (Shh) induction of alkaline phosphatase production in C3H10T1/2 mouse embryonic fibroblast cells.The ED50 for this effect is typically 1.5 - 7.5 µg/mL in the presence of 5 µg/mL rmShh.
• Molecular Mass: Recombinant Mouse Hhip has a calculated MW of 76 kDa & 73 kDa. In SDS-PAGE migrates as 80-90 kDa and 58-64 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.
• Tag: Non

Gene Information

• Gene Name: Hhip Hedgehog-interacting protein [ Mus musculus ]
• Official Symbol: Hhip
• Synonyms: HHIP; Hedgehog-interacting protein; hedgehog-interacting protein; Hhip1
• Gene ID: 15245
• mRNA Refseq: NM_020259
• Protein Refseq: NP_064655