Active Recombinant Mouse Grem1 Protein, His-tagged

• Cat.No.: Grem1-824M
• Product Overview: Recombinant mouse GREM1 (O70326) (Met1-Asp184) was fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 1-184 a.a.
• Predicted N Terminal: Lys 25
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM EDTA, 3mM DTT1, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its ability to inhibit recombinant human BMP4 induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1-7 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
• Molecular Mass: The recombinant mouse GREM1 consists of 171 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Grem1 gremlin 1 [ Mus musculus ]
• Official Symbol: Grem1
• Synonyms: GREM1; gremlin 1; gremlin-1; down-regulated in v-mos-transformed cells; cysteine knot superfamily 1, BMP antagonist 1; down-regulated in Mos-transformed cells protein; ld; Drm; Grem; Cktsf1b1
• Gene ID: 23892
• mRNA Refseq: NM_011824
• Protein Refseq: NP_035954
• Function: cytokine activity