Active Recombinant Mouse Grem1 Protein, His-tagged

• Cat.No.: Grem1-1011M
• Product Overview: Recombinant Mouse Grem1 (Accession # O70326) Lys25-Asp184, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Lys25
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells.The ED50 for this effect is typically 0.025-0.15 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4.
• Molecular Mass: Recombinant Mouse Grem1, His-tagged has a calculated MW of 19.6 kDa. In SDS-PAGE migrates as 23-31 kDa, reducing conditions.
• Protein length: Lys25-Asp184
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Grem1 gremlin 1 [ Mus musculus ]
• Official Symbol: Grem1
• Synonyms: GREM1; gremlin 1; gremlin-1; down-regulated in v-mos-transformed cells; cysteine knot superfamily 1, BMP antagonist 1; down-regulated in Mos-transformed cells protein; ld; Drm; Grem; Cktsf1b1
• Gene ID: 23892
• mRNA Refseq: NM_011824
• Protein Refseq: NP_035954