Active Recombinant Mouse Glo1 Protein, His-tagged

• Cat.No.: Glo1-62M
• Product Overview: Recombinant mouse Glo1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Description: Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Bio-activity: Specific activity is >210 units/mg, and is defined as the amount of enzyme that will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25 centigrade.
• Molecular Mass: 21.8kDa (192aa)28-40kDa (SDS-PAGE under reducing conditions)
• AA Sequence: MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTAWTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKIATIILEHHHH HH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Glo1 glyoxylase 1 complex [ Mus musculus ]
• Official Symbol: Glo1
• Synonyms: GLO1; glyoxylase 1 complex; Glo-1
• Gene ID: 14648