Recombinant Human GlyoxalaseⅠ

• Cat.No.: GLO1-125H
• Product Overview: Recombinant human GLO1 protein was expressed in E.coli and purified by using conventional chromatography techniques, 20.7 kDa (184 aa).

Specification

• Cat. No.: GLO1-125H
• Description: Glyoxalase I, also known as GLO1, belongs to the glyoxalaseⅠfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated.
• Sequences of amino acids: MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
• Purity: > 90% by SDS - PAGE
• Form: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
• Molecular Weight: 20.7 kDa (184 aa), confirmed by MALDI-TOF
• Concentration: 1 mg/ml (determined by Bradford assay)
• Biological activity: Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25C. Specific activity was expressed in units/mg of protein.
• Activity Assay: 1. Prepare a 1.5ml reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033%(w/v) reduced glutathine, 0.003% (w/v) bovine serum albumin. 2. Equilibrate to 25C and monitor at A240nm (absorbance) until the value is constant, using a spectrophotometer. 3. Add 50ul of recombinant glyoxalase I solution with various concentrations (0.5ug, 1ug, 2ug) in 1.4ml reaction buffer. 4. Immediately mix by inversion and record the increase at A240nm for 5 minutes.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Tag: Non

Gene Information

• Gene Name: GLO1 glyoxalase I [ Homo sapiens ]
• Synonyms: GLO1; glyoxalase I; GLYI; GLOD1; GLO1; EC 4.4.1.5; OTTHUMP00000016339; lactoyl glutathione lyase; glyoxalase domain containing 1; Aldoketomutase; Methylglyoxalase; Glx I; etone-aldehyde mutase; S-D-lactoylglutathione methylglyoxal lyase; Lactoylglutathione lyase; Ketone-aldehyde mutase
• Gene ID: 2739
• mRNA Refseq: NM_006708
• Protein Refseq: NP_006699.2
• MIM: 138750
• UniProt ID: Q04760
• Chromosome Location: 6p21.3-p21.1
• Pathway: Pyruvate metabolism
• Function: lactoylglutathione lyase activity; lyase activity; metal ion binding; zinc ion binding