Active Recombinant Mouse Fcgr2b protein(Met1-Arg217), His-tagged

• Cat.No.: Fcgr2b-648M
• Product Overview: Recombinant Mouse FCGR2B (NP_001070657.1)(Met 1-Arg 217) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg217
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Immobilized Mouse FcγRIIB / CD32b recombinant protein at 10 μg/mL can bind biotinylated human IgG1. The EC50 is 0.13-0.29 μg/mL. 2. Loaded Mouse FcγRIIB / CD32b recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.1 μM as determined in a BLI assay (ForteBio Octet Red384).
• Molecular Mass: The secreted recombinant mouse FCGR2B consists of 189 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR2B is approximately 34 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgr2b Fc receptor, IgG, low affinity IIb [ Mus musculus ]
• Official Symbol: Fcgr2b
• Synonyms: FCGR2B; Fc receptor, IgG, low affinity IIb; low affinity immunoglobulin gamma Fc region receptor II-b; fcRII; FcgammaRIIB; Fc-gamma-RII; fc-gamma RII; Fc gamma RIIB; fc-gamma-RIIB; fc gamma receptor IIB; lymphocyte antigen 17; igG Fc receptor II beta; low affinity immunoglobulin gamma Fc region receptor II; CD32; Fcgr2; Fcr-2; Fcr-3; Ly-17; LyM-1; Lym-1; FcgRII; Fcgr2a; Ly-m20; AI528646; Fc[g]RII; F630109E10Rik
• Gene ID: 14130
• mRNA Refseq: NM_001077189
• Protein Refseq: NP_001070657