Active Recombinant Mouse Epor protein, His-tagged

• Cat.No.: Epor-2290M
• Product Overview: Recombinant Mouse Epor protein(NP_034279.3)(Met1-Pro249), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO.2. Measured by its binding ability in a functional ELISA.3. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His.The EC50 of biotinylated mouse EPO-His is 34.5-80.6ng/mL.
• Molecular Mass: The recombinant mouse EPOR comprises 236 amino acids with a predicted molecular mass of 26.2 kDa. As a result of glycosylation, the apparent mplecular mass of rmEPOR is approximately 30-35 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Pro249
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Epor erythropoietin receptor [ Mus musculus ]
• Official Symbol: Epor
• Synonyms: EPOR; erythropoietin receptor; EPO-R
• Gene ID: 13857
• mRNA Refseq: NM_010149
• Protein Refseq: NP_034279