Active Recombinant Mouse Enpp7, His-tagged

• Cat.No.: Enpp7-651M
• Product Overview: Recombinant Mouse Enpp7 (Accession # NP_001025462) Ala22-Gln421, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 22-421 a.a.
• Predicted N Terminal: Ala22
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL and the phosphate is detected/measured by a Malachite Green Phosphate Detection Kit .The specific activity is >13,000 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Enpp7, His-tagged has a calculated MW of 47 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Enpp7 ectonucleotide pyrophosphatase/phosphodiesterase 7 [ Mus musculus ]
• Official Symbol: Enpp7
• Synonyms: ENPP7; ectonucleotide pyrophosphatase/phosphodiesterase 7; Gm254; Alk-SMase
• Gene ID: 404708