Recombinant Rat Enpp7 protein, His-tagged

• Cat.No.: Enpp7-7724R
• Product Overview: Recombinant Rat Enpp7 aa. (Gly22~Val439) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 51kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Gly22~Val439
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Enpp7 ectonucleotide pyrophosphatase/phosphodiesterase 7 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Enpp7
• Synonyms: E-NPP 7; E-NPP7; NPP-7; alk-SMase; alkaline sphingomyelin phosphodiesterase; intestinal alkaline sphingomyelinase; sphingomyelinase, intestinal alkaline; ectonucleotide pyrophosphatase/phosphodiesterase family member 7
• Gene ID: 303729
• mRNA Refseq: NM_001012466.1
• Protein Refseq: NP_001012484.1
• UniProt ID: Q5EZ72