Active Recombinant Mouse Ectonucleotide Pyrophosphatase/Phosphodiesterase 7, His-tagged

• Cat.No.: Enpp7-452M
• Product Overview: RecombinantMouse Enpp7protein was expressed in Murine myeloma cell line. Ala22-Gln421,with a C terminal 10 His tag.

Specification

• Cat. No.: Enpp7-452M
• Description: Enpp7is expressedin the intestines and in bile. It shares 30 36% homology with the members ofthe nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharingno similarities with neutral or acid SMase. Its main function is thedigestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramideand phosphorylcholine. Enpp7 is reported tohydrolyse and inactivate plateletactivating factor (PAF) by a phospholipase Ctypeactivity. Studies show a decrease in Enpp7 activityin human colorectal adenocarcinomas and human colorectal carcinomas, whichindicate a potential role of Enpp7 in human coloncancer.
• Source: Mammalian cells
• Form: Suppliedas a 0.2 um filtered solution in Tris and NaCl.
• N-terminalSequence: Ala22
• MolecularWeight: 47 kDa
• Activity: Measuredby its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine.The phosphorylcholine is cleaved by rhAlkaline Phosphatase/ALPL and thephosphate is detected or measured by a Malachite Green Phosphate DetectionKit. The specific activity is > 13000 pmoles/min/μg, as measured under thedescribed conditions. See Activity Assay Protocol.
• Endotoxin Level: <1.0EU per 1 μg of the protein by the LAL method.
• Purity: >95%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Storage: Use amanual defrost freezer and avoid repeated freezethaw cycles. 6 monthsfrom date of receipt, 20 to 70°C as supplied. 3 months, 20 to 70°C understerile conditions after opening.
• Protein length: Ala22-Gln421

Gene Information

• Gene Name: Enpp7 ectonucleotidepyrophosphatase/phosphodiesterase 7 [ Mus musculus ]
• Official Symbol: Enpp7
• Synonyms: Enpp7;ectonucleotide pyrophosphatase/phosphodiesterase 7; Gm254; Alk-SMase; ectonucleotidepyrophosphatase/phosphodiesterase family member 7; EC 3.1.4.12
• Gene ID: 238011
• mRNA Refseq: NM_001030291
• Protein Refseq: NP_001025462
• UniProt ID: Q3TIW9
• Chromosome Location: 11
• Pathway: Metabolic pathways;Sphingolipid metabolism
• Function: NOT nucleotidaseactivity; sphingomyelin phosphodiesterase activity

Reviews

03/12/2020

With their extensive knowledge and expertise, I am assured that any challenges or issues I may encounter during my research will be efficiently resolved.

10/16/2017

I am highly impressed with the ENPP7 protein as it is of exceptionally high quality, and I am confident that it will perfectly meet the specific requirements of my experiments.

01/09/2016

Such dedicated support makes me feel confident in my choice of using the ENPP7 protein for my experiments.

Q&As

Are there diagnostic applications of ENPP7 in clinical settings?

ENPP7 levels or activity may serve as biomarkers in diagnosing certain metabolic disorders or assessing cardiovascular risk.

How is ENPP7 expression regulated in the human body?

The expression of ENPP7 is regulated by various factors, including diet, hormones, and genetic factors.

Are there any genetic mutations associated with ENPP7 and diseases?

Genetic variations in the ENPP7 gene have been linked to certain diseases, including metabolic disorders and cardiovascular conditions.

How is ENPP7 protein linked to atherosclerosis?

ENPP7 is thought to play a protective role in atherosclerosis by influencing lipid profiles and reducing the risk of plaque formation in blood vessels.

Can ENPP7 be a potential therapeutic target for cardiovascular diseases?

Research suggests that modulating ENPP7 activity may have therapeutic potential for managing cardiovascular diseases, but further studies are needed.