Active Recombinant Mouse Dkk3 Protein

• Cat.No.: Dkk3-7394M
• Product Overview: Recombinant mouse DKK-3 protein without tag was expressed in CHO cells.

Specification

• Species: Mouse
• Source: CHO
• Description: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
• Form: Lyophilized
• Bio-activity: Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 E1 cells. The expected ED50 for this effect is 2.0–4.0 ng/mL.
• Molecular Mass: 36.3 kDa (329aa)
• AA Sequence: APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALREPAAAAAALLGGEEI
• Purity: > 98 % by SDS-PAGE and HPLC
• Stability: Shelf life: one year from despatch.
• Storage: Store lyophilized at 2-8 centigrade for 6 months or at -20 centigrade long term.
  After reconstitution store the antibody undiluted at 2-8 centigrade for one month or (in aliquots) at -20 centigrade long term.
  Avoid repeated freezing and thawing.
• Reconstitution: We recommended a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at 4 centigrade for one week or at –20 centigrade for future use.

Gene Information

• Gene Name: Dkk3 dickkopf WNT signaling pathway inhibitor 3 [ Mus musculus (house mouse) ]
• Official Symbol: Dkk3
• Synonyms: Dkk3; dickkopf WNT signaling pathway inhibitor 3; dkk-3; C87148; mDkk-3; AW061014; dickkopf-related protein 3; dickkopf homolog 3; dickkopf-3
• Gene ID: 50781
• mRNA Refseq: NM_001360257
• Protein Refseq: NP_001347186
• UniProt ID: Q9QUN9