Recombinant Rat Dkk3 protein, His & T7-tagged

• Cat.No.: Dkk3-8014R
• Product Overview: Recombinant Rat Dkk3 aa. (Pro41~Ile348 (Accession # B1H219)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His&T7
• Protein Length: Pro41~Ile348
• Form: Freeze-dried powder
• Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 4.5.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Publications:
  Use of tumor suppressor genes of naked mole rats for human cancer treatment (2023)

Gene Information

• Gene Name: Dkk3 dickkopf WNT signaling pathway inhibitor 3 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Dkk3
• Synonyms: Dkk3; dickkopf WNT signaling pathway inhibitor 3; p29; Reic; dickkopf-related protein 3; dickkopf 3 homolog; dickkopf homolog 3
• Gene ID: 171548
• mRNA Refseq: NM_138519.2
• Protein Refseq: NP_612528.2
• UniProt ID: B1H219