Active Recombinant Mouse Chst1 Protein, His-tagged

• Cat.No.: Chst1-751M
• Product Overview: Recombinant Mouse Chst1 (Accession # NP_076339) Arg24-Leu411, fused with an N-terminal 6-His tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Species: Mouse
• Source: CHO
• Tag: His
• Protein Length: Arg24-Leu411
• Predicted N Terminal: His
• Form: Supplied as a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to transfer sulfate from PAPS to alpha -Lactose.The specific activity is >500 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Chst1, His-tagged has a calculated MW of 45 kDa. In SDS-PAGE migrates as 50-60 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Chst1 carbohydrate (keratan sulfate Gal-6) sulfotransferase 1 [ Mus musculus ]
• Official Symbol: Chst1
• Synonyms: CHST1; carbohydrate (keratan sulfate Gal-6) sulfotransferase 1; carbohydrate sulfotransferase 1; KSST; KS6ST; keratan sulfate Gal-6 sulfotransferase; N-acetylglucosamine-6-O-sulfotransferase 1; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1; C6ST; Gst1; GST-1; AW125896; KSGAL6ST; 2610008E20Rik
• Gene ID: 76969
• mRNA Refseq: NM_023850
• Protein Refseq: NP_076339