Active Recombinant Mouse Chemokine (C-C motif) Ligand 9

• Cat.No.: Ccl9-403M
• Product Overview: Recombinantmouse Ccl9 protein was expressed in E. coli. Gln22-Gln122.

Specification

• Cat. No.: Ccl9-403M
• Description: Mouse Ccl9 (alsoMIP1γ and MRP2) is an 11 kDa, secreted, monomeric polypeptide that belongs tothe β (or CC) intercrine family of chemokines. Based on its activity andamino acid (aa) sequence, it is further classified as a member of the NC6 orsixcysteine containing CC subfamily of chemokines.
• Source: E. coli
• Form: Lyophilized from a0.2 μm filtered solution in Acetonitrile and TAF with BSA as a carrierprotein.
• N-terminal Sequence: Gln22
• Molecular Weight: 11.5 kDa
• Activity: Measured by itsability to chemoattract THP-1 human acute monocytic leukemia cells. The ED50for this effect is typically 0.2-0.6 μg/mL. Measured by its ability tochemoattract human CCR1 transfected BaF3 mouse pro-B cells. The ED50for this effect is typically 0.002-0.01 μg/mL.
• Endotoxin Level: <1.0 EU per 1 μgof the protein by the LAL method.
• Purity: >97%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstitute at 25μg/mL in sterile PBS that containing at least 0.1% human or bovine serumalbumin.
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied. 1month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to-70°C under sterile conditions after reconstitution.
• Tag: Non
• Protein length: 22-122 a.a.

Gene Information

• Gene Name: Ccl9 chemokine (C-C motif)ligand 9 [ Mus musculus ]
• Official Symbol: Ccl9
• Synonyms: Ccl9; chemokine(C-C motif) ligand 9; CCF18; MRP-2; Scya9; Scya10; OTTMUSP00000000947;MIP-1 gamma; C-C motif chemokine 9; small induciblecytokine A9; small inducible cytokine A10; macrophage inflammatory protein1-gamma; macrophage inflammatory protein-ratated protein 2; MIP-1-gamma;Mrp2; Small-inducible cytokine A9
• Gene ID: 20308
• mRNA Refseq: NM_011338
• Protein Refseq: NP_035468
• UniProt ID: P51670
• Chromosome Location: 11
• Pathway: Chemokine signalingpathway; Class A/1 (Rhodopsin-like receptors); Cytokine-cytokine receptorinteraction; GPCR ligand binding; GPCR downstream signaling; G alpha (i)signaling events; G alpha (q) signaling events; Peptide ligand-bindingreceptors; Signal Transduction; Signaling by GPCR
• Function: chemokine activity; cytokineactivity