Active Recombinant Mouse Ccl9 Protein

• Cat.No.: Ccl9-01M
• Product Overview: Recombinant Mouse Ccl9 Protein without tag was expressed in E. coli.

Specification

• Description: Predicted to enable CCR1 chemokine receptor binding activity; chemoattractant activity; and chemokine activity. Acts upstream of or within negative regulation of myoblast differentiation. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; brain; eye; genitourinary system; and limb. Orthologous to several human genes including CCL23 (C-C motif chemokine ligand 23).
• Source: E. coli
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: Measured by its ability to chemoattract human CCR1 transfected BaF3 mouse proB cells. The ED50 for this effect is typically 0.002-0.01 μg/mL.
• Molecular Mass: Approximately 11.5 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids.
• Protein length: 101 amino acids
• AA Sequence: QITHATETKEVQSSLKAQQGLEIEMFHMGFQDSSDCCLSYNSRIQCSRFIGYFPTSGGCTRPGIIFISKRGFQVCANPSDRRVQRCIERLEQNSQPRTYKQ
• Endotoxin: Less than 1 EU/μg of rMuCCL9/10 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 or -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Ccl9 chemokine (C-C motif) ligand 9 [ Mus musculus (house mouse) ]
• Official Symbol: Ccl9
• Synonyms: Ccl9; chemokine (C-C motif) ligand 9; CCF18; MRP-2; Scya9; Scya10; C-C motif chemokine 9; MIP-1 gamma; macrophage inflammatory protein 1-gamma; macrophage inflammatory protein-related protein 2; small inducible cytokine A10; small inducible cytokine A9
• Gene ID: 20308
• mRNA Refseq: NM_011338
• Protein Refseq: NP_035468
• UniProt ID: P51670