Active Recombinant Mouse Ces1g Protein (19-565aa), C-His-tagged

• Cat.No.: Ces1g-12M
• Product Overview: Recombinant mouse Carboxylesterase 1/CES1 (19-565aa), fused to His-tag at Cterminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 19-565aa
• Description: This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. The expression and activity of this gene is age-related but independent of growth hormone level. This gene is clustered with Ces7 and Ces3 on chromosome 8.
• Form: Liquid
• Bio-activity: > 2000 pmol/min/μg and is defined as the amount of enzyme that hydrolyze 1 pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37 centigrade.
• Molecular Mass: 61.9 kDa (556 aa)
• AA Sequence: HPSLPPVVHTVHGKVLGKYVTLEGFSQPVAVFLGVPFAKPPLGSLRFAPPEPAEPWSFVKHTTSYPPLCYQNPEAALRLAELFTNQRKIIPHKFSEDCLYLNIYTPADLTQNSRLPVMVWIHGGGLVIDGASTYDGVPLAVHENVVVVVIQYRLGIWGFFSTEDEHSRGNWGHLDQVAALHWVQDNIANFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAIAQSSVIFNPCLFGRAARPLAKKIAALAGCKTTTSAAMVHCLRQKTEDELLEVSLKMKFGTVDFLGDPRESYPFLPTVIDGVLLPKAPEEILAEKSFNTVPYMVGINKHEFGWIIPMFLDFPLSERKLDQKTAASILWQAYPILNISEKLIPAAIEKYLGGTEDPATMTDLFLDLIGDIMFGVPSVIVSRSHRDAGAPTYMYEYQYRPSFVSDDRPQELLGDHADELFSVWGAPFLKEGASEEEINLSKMVMKFWANFARNGNPNGEGLPHWPEYDQKEGYLQIGVPAQAAHRLKDKEVDFWTELRAKETAERSSHREHVEL
• Endotoxin: < 1.0 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Ces1g carboxylesterase 1G [ Mus musculus (house mouse) ]
• Official Symbol: Ces1g
• Synonyms: Ces1g; carboxylesterase 1G; liver carboxylesterase 1; ES-x; acyl-coenzyme A:cholesterol acyltransferase; carboxylesterase 1; cholesteryl ester hydrolase; EC 3.1.1.1; EC 3.1.1.13; CEH; Ces1; Ces-1; Ses-1
• Gene ID: 12623
• mRNA Refseq: NM_021456
• Protein Refseq: NP_067431
• UniProt ID: Q8VCC2