Active Recombinant Mouse Cdh6 Protein, His-tagged

• Cat.No.: Cdh6-593M
• Product Overview: Recombinant Mouse Cdh6 (Accession # NP_031692) Met1-Ala615, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Asn22 (pro-protein) & Ser54 (mature protein)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells.The ED50 for this effect is typically 0.2 - 1.0 µg/mL after a 30 minutes incubation at 37 °C.Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Cdh6, His-tagged has a calculated MW of 67.0 kDa (pro-protein) & 63.3 kDa (mature protein). In SDS-PAGE migrates as 90-105 kDa, reducing conditions.
• Protein length: Met1-Ala615
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Cdh6 cadherin 6 [ Mus musculus ]
• Official Symbol: Cdh6
• Synonyms: CDH6; cadherin 6; cadherin-6; kidney cadherin; cad6; K-cadherin; MGC129338; MGC129360
• Gene ID: 12563
• mRNA Refseq: NM_007666
• Protein Refseq: NP_031692