Active Recombinant Mouse CD3E & CD3G protein, His &Flag-tagged
Cat.No. : | CD3E & CD3G-3038M |
Product Overview : | Recombinant Mouse CD3E&CD3G protein(Asp 23-Asp 108 (CD3E) & Gln 23-Ser 116 (CD3G)), fused with His and Flag tag, was expressed in HEK293. |
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Species : | Mouse |
Source : | HEK293 |
Tag : | Flag&His |
Protein Length : | Asp 23-Asp 108 (CD3E) & Gln 23-Ser 116 (CD3G) |
Form : | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag at 1 μg/mL (100 μL/well) can bind CD3e Monoclonal Antibody (145-2C11) with a linear range of 0.1-4ng/mL. |
Molecular Mass : | Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 15.1 kDa (CD3E) and 15.2 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3G is fused with a flag tag at the C-terminus. As a result of glycosylation, the protein migrates as 19-22 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE). |
Endotoxin : | Less than 1.0 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Storage : | For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name | Cd3e CD3 antigen, epsilon polypeptide [ Mus musculus ] |
Official Symbol | CD3E |
Synonyms | CD3E; CD3 antigen, epsilon polypeptide; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3; T3e; AI504783; CD3epsilon; |
Gene ID | 12501 |
mRNA Refseq | NM_007648 |
Protein Refseq | NP_031674 |
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CD3E & CD3G-3038M | Active Recombinant Mouse CD3E & CD3G protein, His &Flag-tagged | +Inquiry |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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