Active Recombinant Mouse CD276 protein, Fc-tagged

• Cat.No.: CD276-2905M
• Product Overview: Recombinant Mouse CD276 protein(Val 29-Phe 244), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse B7-H3, Fc Tag at 1 μg/mL (100 μL/well) can bind Anti-B7-H3 Antibody, Mouse IgG1 with a linear range of 4-63 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 50.1 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Val 29-Phe 244
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd276 CD276 antigen [ Mus musculus ]
• Official Symbol: CD276
• Synonyms: CD276; CD276 antigen; B7-H3; B7 homolog 3; costimulatory molecule; B7h3; B7RP-2; AU016588; 6030411F23Rik
• Gene ID: 102657
• mRNA Refseq: NM_133983
• Protein Refseq: NP_598744