Active Recombinant Human CD276 Protein, His-tagged

• Cat.No.: CD276-757H
• Product Overview: Recombinant Human CD276 (Accession # NP_079516) Leu29-Pro245, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Leu29
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using mouse CD4+ cells in the presence of anti-CD3.The ED50 for this effect is typically 3-12 µg/mL.
• Molecular Mass: Recombinant Human CD276, His-tagged has a calculated MW of 24.8 kDa. In SDS-PAGE migrates as 40-45 kDa, reducing conditions.
• Protein length: Leu29-Pro245
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 25 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: CD276 CD276 molecule [ Homo sapiens ]
• Official Symbol: CD276
• Synonyms: CD276; CD276 molecule; CD276 antigen; B7 H3; B7H3; B7RP 2; B7 homolog 3; costimulatory molecule; B7-H3; B7RP-2; 4Ig-B7-H3
• Gene ID: 80381
• mRNA Refseq: NM_001024736
• Protein Refseq: NP_001019907
• MIM: 605715
• UniProt ID: Q5ZPR3
• Chromosome Location: 15q23-q24