Active Recombinant Mouse Car2 Protein, His-tagged

Cat.No. : Car2-886M
Product Overview : Recombinant mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.
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Species : Mouse
Source : E.coli
Tag : His
Protein Length : 1-260 a.a.
Predicted N Terminal : Ser 2
Form : Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.51, 5%~8% trehalose and mannitol.
Bio-activity : Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.
Molecular Mass : The recombinant mouse CA2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >96 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name Car2 carbonic anhydrase 2 [ Mus musculus ]
Official Symbol Car2
Synonyms Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; CA II; CA-II; carbonate dehydratase II; carbonic anhydrase II
Gene ID 12349
mRNA Refseq NM_009801
Protein Refseq NP_033931
MIM
UniProt ID P00920
Chromosome Location 3 A1; 3 3.23 cM
Pathway Collecting duct acid secretion, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Pancreatic secretion, conserved biosystem
Function arylesterase activity; carbonate dehydratase activity; metal ion binding

Not For Human Consumption!

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