Active Recombinant Mouse Car2 Protein, His-tagged
Cat.No. : | Car2-886M |
Product Overview : | Recombinant mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag. |
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Species : | Mouse |
Source : | E.coli |
Tag : | His |
Protein Length : | 1-260 a.a. |
Predicted N Terminal : | Ser 2 |
Form : | Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.51, 5%~8% trehalose and mannitol. |
Bio-activity : | Measured by its esterase activity. The specific activity is >100 pmoles/min/μg. |
Molecular Mass : | The recombinant mouse CA2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | >96 % as determined by SDS-PAGE. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70ºC. |
Storage : | Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name | Car2 carbonic anhydrase 2 [ Mus musculus ] |
Official Symbol | Car2 |
Synonyms | Ca2; CAII; Car-2; Ltw-5; Lvtw-5; AI131712; CA II; CA-II; carbonate dehydratase II; carbonic anhydrase II |
Gene ID | 12349 |
mRNA Refseq | NM_009801 |
Protein Refseq | NP_033931 |
MIM | |
UniProt ID | P00920 |
Chromosome Location | 3 A1; 3 3.23 cM |
Pathway | Collecting duct acid secretion, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Pancreatic secretion, conserved biosystem |
Function | arylesterase activity; carbonate dehydratase activity; metal ion binding |
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◆ Cell & Tissue Lysates | ||
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Not For Human Consumption!
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