Active Recombinant Mouse Car14 Protein, His-tagged

• Cat.No.: Car14-822M
• Product Overview: Recombinant extracellular domain of mouse Car14 (NP_035927.1) (Met 1-Met 290) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-290 a.a.
• Predicted N Terminal: Ala 16
• Form: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.51, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its esterase activity. The specific activity is >400 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 0.8 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
• Molecular Mass: The recombinant mouse CA14 consists of 286 amino acids and has a calculated molecular mass of 32.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 45-48 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Car14 carbonic anhydrase 14 [ Mus musculus ]
• Official Symbol: Car14
• Synonyms: Ca14; AW536446; CA XIV; CA-XIV; carbonate dehydratase XIV; carbonic anhydrase XIV
• Gene ID: 23831
• mRNA Refseq: NM_011797
• Protein Refseq: NP_035927
• UniProt ID: Q9WVT6
• Chromosome Location: 3; 3 F2.1
• Pathway: Metabolism, organism-specific biosystem; Nitrogen metabolism, organism-specific biosystem; Nitrogen metabolism, conserved biosystem
• Function: carbonate dehydratase activity; lyase activity; metal ion binding