Active Recombinant Mouse Bsg protein, His&hFc-tagged

• Cat.No.: Bsg-2268M
• Product Overview: Recombinant Mouse Bsg protein(NP_001070652.1)(Met1-Arg209), fused with His and hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: Met1-Arg209
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Using the Octet RED System, the affinity constant (Kd) of mouse CD147-Fc bound to Human PPIA-His was 0.4 μM.
• Molecular Mass: The secreted recombinant mouse BSG/Fc is a disulfide-linked homodimer The reduced monomer comprises 436 amino acids and has a predicted molecular mass of 48.6 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Bsg basigin [ Mus musculus ]
• Official Symbol: Bsg
• Synonyms: BSG; basigin; gp 42; HT7 antigen; neurothelin; 5A11/Basigin; membrane glycoprotein gp42; basic immunoglobulin superfamily; HT-7; CD147; EMMPRIN; AI115436; AI325119
• Gene ID: 12215
• mRNA Refseq: NM_001077184
• Protein Refseq: NP_001070652