Active Recombinant Mouse Bid protein(Met1-Asp195), His&GST-tagged

• Cat.No.: Bid-4029M
• Product Overview: Recombinant Mouse BID (EDK99650.1) (Met 1-Asp 195) was expressed in E. coli, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His&GST
• Protein length: Met1-Asp195
• Form: Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.01 ng/mL. 3. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.
• Molecular Mass: The recombinant mouse BID/GST chimera consisting of 435 amino acids and has a calculated molecular mass of 50 kDa. It migrates as a 47 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Bid BH3 interacting domain death agonist [ Mus musculus ]
• Official Symbol: Bid
• Synonyms: BID; BH3 interacting domain death agonist; BH3-interacting domain death agonist; p22 BID; AI875481; AU022477; 2700049M22Rik
• Gene ID: 12122
• mRNA Refseq: NM_007544
• Protein Refseq: NP_031570