Active Recombinant Mouse Artemin

• Cat.No.: Artn-578M
• Product Overview: Recombinant Mouse Artnprotein was expressed in E. coli. Ala112-Gly224.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• ProteinLength: 112-224 a.a.
• Description: Artn is a member ofthe Glia CellDerived Neurotrophic factor (GDNF) family ligands, which includeGDNF, Persephin, Artn, and Neurturin. GDNF family ligands are distant membersof the Transforming Growth Factor β (TGFβ) superfamily. Similar to other TGFβfamily proteins, Artn is synthesized as a large precursor protein that iscleaved at the dibasic cleavage site (RXXR) to release the carboxy terminal domain.
• Form: Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
• N-terminalSequence: Ala112
• Molecular Weight: 12 kDa (monomer)
• Activity: Measured in a cellproliferation assay using SHSY5Y human neuroblastoma cells. The ED50for this effect is typically 2-8 ng/mL. Measured by its binding ability in afunctional ELISA. Immobilized recombinant human GFRα3 Fc Chimera at 2 μg/mL(100 μL/well) can bind Recombinant Mouse Artemin with a linear range of 0.08-10ng/mL.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• Purity: > 97%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mL in sterile4 mM HCI containing at least 0.1% human or bovine serum albumin.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Artn artemin [ Mus musculus]
• Official Symbol: Artn
• Synonyms: Artn; artemin;neublastin
• Gene ID: 11876
• mRNA Refseq: NM_009711
• Protein Refseq: NP_033841
• Chromosome Location: 4; 4 D1
• Pathway: Axon guidance;Developmental Biology; NCAM signaling for neurite out-growth; NCAM1interactions
• Function: glial cellline-derived neurotrophic factor receptor binding; growth factor activity;protein binding; receptor binding