Recombinant Human ARTN protein, hFc-tagged

• Cat.No.: ARTN-5643H
• Product Overview: Recombinant Human ARTN protein(Q5T4W7-1)(Ala108-Gly220), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• ProteinLength: Ala108-Gly220
• Tag: N-hFc
• Form: Lyophilized from 0.22μm filtered solution in 4mM HCl (pH 2.8). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human GFRA3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 92.5ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 39.3 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCl (pH2.8).

Gene Information

• Gene Name: ARTN artemin [ Homo sapiens ]
• Official Symbol: ARTN
• Synonyms: ARTN; artemin; ENOVIN; EVN; NBN; neublastin; neurotrophic factor
• Gene ID: 9048
• mRNA Refseq: NM_001136215
• Protein Refseq: NP_001129687
• MIM: 603886
• UniProt ID: Q5T4W7