Active Recombinant Mouse Alcam, Fc Chimera

• Cat.No.: Alcam-232M
• Product Overview: Recombinant Mouse Alcam (Accession # AAC06342) was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Predicted N Terminal: Trp28
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to block adhesion of HuT 78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6 Fc Chimera .Recombinant Mouse ALCAM/CD166 Fc Chimera at 10 µg/mL (50 µL/well) blocked 100% of HUT-78 cell adhesion (1 x 10^6 cells/mL, 50 µL/well) to immobilized recombinant human CD6 Fc Chimera (10 µg/mL, 100 µL/well). Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: Recombinant Mouse Alcam, Fc Chimera has a calculated MW of 82 kDa (monomer). In SDS-PAGE migrates as 114-122 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Alcam activated leukocyte cell adhesion molecule [ Mus musculus ]
• Official Symbol: Alcam
• Synonyms: ALCAM; activated leukocyte cell adhesion molecule; CD166 antigen; BEN; SC1; MuSC; CD166; AI853494; DM-GRASP; MGC27910
• Gene ID: 11658
• mRNA Refseq: NM_009655
• Protein Refseq: NP_033785