Active Recombinant Mouse Ahsg protein(Met1-Ile345), His-tagged

• Cat.No.: Ahsg-705M
• Product Overview: Recombinant Mouse AHSG (NP_038493.1) precursor (Met1-Ile 345) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• ProteinLength: Met1-Ile345
• Form: Lyophilized from sterile 25mM Tris, 0.15mM NaCl, pH 7.8. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <200 nM.
• Molecular Mass: The secreted recombinant mouse AHSG consists of 338 amino acids and has a predicted molecular mass of 36.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm AHSG is approximately 50 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ahsg alpha-2-HS-glycoprotein [ Mus musculus ]
• Official Symbol: Ahsg
• Synonyms: AHSG; alpha-2-HS-glycoprotein; countertrypin; fetuin-A
• Gene ID: 11625
• mRNA Refseq: NM_013465
• Protein Refseq: NP_038493