Recombinant Guinea pig Ahsg Protein, His-tagged

• Cat.No.: Ahsg-102G
• Product Overview: Recombinant Guinea pig Ahsg Protein (Thr19-IIe358) with N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including endocytosis, brain development, and the formation of bone tissue.
• Source: E. coli
• Species: Guinea pig
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 40 kDa
  Accurate Molecular Mass: 49 kDa
• Protein length: Thr19-IIe358
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Ahsg alpha-2-HS-glycoprotein [ Cavia porcellus (domestic guinea pig) ]
• Official Symbol: Ahsg
• Synonyms: AHSG; alpha-2-HS-glycoprotein; fetuin-A; alpha-2-HS glycoprotein; FETUA
• Gene ID: 100135479
• mRNA Refseq: NM_001172913
• Protein Refseq: NP_001166384
• UniProt ID: O70159

Reviews

06/03/2022

Absolutely loving the lab protein – a real game changer!

10/19/2021

Great value for the quality, and the speedy delivery was a pleasant surprise!

08/16/2020

Thumbs up for the protein, it's become my new favorite.

02/27/2019

Can't help but smile at the results from the product!

12/11/2018

Balanced pricing, superior quality, and top-level customer assistance.

Q&As

How can proteomics approaches be used to study the protein-protein interactions involving AHSG in a global context?

Techniques like co-immunoprecipitation coupled with mass spectrometry can reveal AHSG's interactome, shedding light on its broader functional networks.

Explain the potential diagnostic applications of AHSG in conditions such as chronic kidney disease and diabetes?

AHSG levels may be altered in these conditions, making it a potential biomarker for disease progression or severity.

Explain the potential role of AHSG in mediating endocrine functions and its connection to insulin resistance?

AHSG's interaction with insulin and adipokines could impact insulin sensitivity and metabolism, contributing to insulin resistance in conditions like type 2 diabetes.

Discuss the interactions between AHSG and other proteins, particularly its relationship with fetuin-B and fetuin-A?

AHSG can form complexes with fetuin-B and fetuin-A, which have implications for calcium homeostasis, insulin signaling, and other physiological processes.

Explain AHSG's role in modulating immune responses and its potential relevance to autoimmune disorders?

AHSG can interact with immune cells and cytokines, potentially influencing immune responses. Dysregulated AHSG levels might contribute to autoimmune diseases.

How might AHSG's interactions with circulating proteins influence its functions and its potential clinical relevance?

AHSG's interactions with proteins like albumin and hormones can impact its bioavailability and activity, influencing physiological processes.

Describe the biochemical methods used to analyze AHSG's post-translational modifications, including glycosylation.

Techniques such as mass spectrometry, lectin affinity chromatography, and glycan analysis can provide insights into AHSG's glycosylation patterns.

How does AHSG regulate calcium homeostasis, and what implications does this have for human health?

AHSG binds to calcium ions and influences their distribution in the body. Dysregulation may impact bone health and contribute to conditions like osteoporosis.

How can researchers isolate and purify AHSG for biochemical studies in the laboratory?

Techniques such as ion exchange chromatography, gel filtration, and affinity chromatography can be used to isolate and purify AHSG from biological samples.

How might genetic variations in the AHSG gene impact its protein expression and functions?

Genetic polymorphisms in the AHSG gene might lead to altered AHSG levels or structural changes, potentially influencing its physiological roles.