Active Recombinant Mouse Agouti Related Protein

• Cat.No.: Agrp-546M
• Product Overview: Recombinant Mouse Agrpprotein was expressed in E. coli. Ser82-Thr131.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Fc
• Protein Length: 82-131 a.a.
• Description: Agrp, the proteinproduct of the Agouti Related Transcript (ART), is a neuroprotein thatregulates energy metabolism and the development of obesity by antagonizing αmelanocytestimulating hormone (α-MSH) action on MC-3 and MC-4 receptors. Agrp is predominantlyexpressed in the hypothalamus and adrenal medulla. Mature mouse Agrp is a 111amino acid (aa) polypeptide; its C-terminal portion contains ten conservedcysteines that form five disulfide bonds. Within the C-terminal region, mouseAgrp shares 80% and 90% aa sequence identity with human and rat Agrp,respectively. It also shares 44% aa sequence identity with Agrp. As with Agrp,the C-terminal cysteine rich region is sufficient for biological activity.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Ser82
• Molecular Weight: 5.7 kDa
• Activity: Measured by itsability to antagonize α-MSHinduced c-AMP accumulation on human MelanocortinReceptor 4 transfected BaF3 mouse proB cells. The ED50 for thiseffect is typically 1-4 μg/mL in the presence of 10 ng/mL of α-MSH.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• Purity: > 95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Agrp agouti related protein [ Musmusculus ]
• Official Symbol: Agrp
• Synonyms: Agrp; agouti relatedprotein; Art;Agrt; agouti-related protein; orexigenic neuropeptide
• Gene ID: 11604
• mRNA Refseq: NM_007427
• Protein Refseq: NP_031453
• UniProt ID: P56473
• Chromosome Location: 8; 8 D1-D2
• Pathway: Adipocytokine signalingpathway; Adipogenesis
• Function: neuropeptide hormoneactivity