Active Recombinant Monkey MET Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: MET-183CAF555
• Product Overview: A DNA sequence encoding the Monkey /rhesus MET (NP_001162100.1) (Met1-Thr932) (Alexa Fluor 555 conjugated) was expressed with the Fc region of human IgG1 at the C-terminus. Monkey and Rhesus MET sequences are identical.

Specification

• Source: HEK293
• Species: Monkey
• Tag: Fc
• Form: Lyophilized
• Bio-activity: Immobilized Cynomolgus HGF at 10 μg/mL (100 μL/well) can bind Cynomolgus MET-Fc, EC50 of Cynomolgus MET-Fc is 0.04-0.09 μg/mL.
• Molecular Mass: The recombinant cynomolgus/rhesus MET comprises 1146 amino acids and has a calculated molecular mass of 128.3 kDa. The apparent molecular mass of it is approximately 100.2 and 42.5 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Thr932， 1146
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: (66.7+31.5) % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.

Gene Information

• Gene Name: METmet proto-oncogene (hepatocyte growth factor receptor) [Macaca mulatta(Rhesus monkey) ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene (hepatocyte growth factor receptor); hepatocyte growth factor receptor; NP_001162100.1; EC 2.7.10.1
• Gene ID: 704562
• mRNA Refseq: NM_001168629
• Protein Refseq: NP_001162100