Recombinant Active Human MET Protein (956-end), N-His-tagged

• Cat.No.: MET-23H
• Product Overview: Recombinant human MET (956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 956-end
• Description: MET is a proto-oncogene that encodes a transmembrane growth factor receptor which is a heterodimer of two disulphide linked chains of 50 kDa (alpha) and 145 kDa (beta). MET is widely expressed in the kidney, brain, lung, skin, and embryonic tissue. Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian cancers and has been shown to be involved in tumor cell migration and invasion.
• Bio-activity: The specific activity of MET was determined to be 24 nmol/min/mg as per actity assay protocol.
• Molecular Mass: Observed MW: ~60 kDa.
  Calculated MW: ~50 kDa.
• Purity: The purity of MET was determined to be > 70 % by densitometry.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: MET met proto-oncogene (hepatocyte growth factor receptor) [ Homo sapiens (human) ]
• Official Symbol: MET
• Synonyms: MET; met proto-oncogene (hepatocyte growth factor receptor); hepatocyte growth factor receptor; HGFR; RCCP2; SF receptor; HGF receptor; oncogene MET; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; AUTS9; c-Met
• Gene ID: 4233
• mRNA Refseq: NM_000245
• Protein Refseq: NP_000236
• MIM: 164860
• UniProt ID: P08581