Active Recombinant Marmoset LAG3 Protein, His-tagged

• Cat.No.: LAG3-387M
• Product Overview: Recombinant Marmoset LAG3 Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 18 - His 449 (Accession # F7HXP9-1). Predicted N-terminus: Ala 18. This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.0 kDa. The protein migrates as 57 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Species: Marmoset
• Source: HEK293
• Tag: His
• Protein Length: Ala 18 - His 449
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Madarex LAG-3 Mab, Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Marmoset LAG3 Protein, His-tagged with a linear range of 10-156 ng/mL (QC tested).
• Molecular Mass: 48.0 kDa
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.