Active Recombinant Cynomolgus LAG3 protein, mFc-tagged, Biotinylated

• Cat.No.: LAG3-380C
• Product Overview: Biotinylated Recombinant Cynomolgus LAG3 protein(Ala 18 - His 449), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: mFc
• Protein Length: 18-449 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus LAG-3, Mouse IgG2a Fc tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG1 with a linear range of 0.2-6 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 73.5 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala18-His449
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.