Active Recombinant Influenza A H5N1 (A/Cambodia/S1211394/2008) HA protein, His-tagged

• Cat.No.: HA-0385H
• Product Overview: Recombinant Influenza A H5N1 (A/Cambodia/S1211394/2008) HA protein(Met1-Gly341)(ADM95445.1), fused with His tag, was expressed in HEK293.

Specification

• Species: Influenza A H5N1 (A/Cambodia/S1211394/2008)
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gly341
• Form: Lyophilized from sterile PBS-2, pH 7.4, 10% glyPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 16-160μg/mL for 1% GRBC.
• Molecular Mass: The secreted recombinant HA1 subunit of influenza A H5N1 HA (A/Cambodia/S1211394/2008(H5N1)) comprises 526 amino acids and has a predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 83 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.