Active Recombinant H3N8 (A/equine/Gansu/7/2008) HA Protein, His-tagged

• Cat.No.: HA-681V
• Product Overview: Recombinant Influenza A virus (A/equine/Gansu/7/2008(H3N8)) hemagglutinin (ACE81938.1) (Met1-Arg344), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: H3N8
• Tag: His
• Predicted N Terminal: Gln 17
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.0, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. 3. Measured by its ability to agglut
• Molecular Mass: The recombinant HA subunit of Influenza A virus (A/equine/Gansu/7/2008(H3N8)) consists 339 amino acids and predicts a molecular mass of 37.9 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-344 a.a.