Active Recombinant Human UBA6, GST-tagged

• Cat.No.: UBA6-149H
• Product Overview: Recombinant Human UBA6 (Accession # A0AVT1) , fused with an N-terminal GST (glutathione S-transferase) tag, was produced in Spodoptera frugiperda, Sf 9 (baculovirus)-derived.

Specification

• Source: Sf 9 insect cells
• Species: Human
• Tag: GST
• Form: Supplied as a solution in HEPES and DTT.
• Bio-activity: Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) concentration of 50-200 nM.
• Molecular Mass: Recombinant Human UBA6, GST-tagged has a calculated MW of 144 kDa.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: UBA6 ubiquitin-like modifier activating enzyme 6 [ Homo sapiens ]
• Official Symbol: UBA6
• Synonyms: UBA6; ubiquitin-like modifier activating enzyme 6; UBE1L2, ubiquitin activating enzyme E1 like 2; ubiquitin-like modifier-activating enzyme 6; FLJ10808; ubiquitin activating enzyme E1; monocyte protein 4; ubiquitin-activating enzyme 6; UBA6, ubiquitin-activating enzyme E1; ubiquitin-activating enzyme E1-like 2; ubiquitin-activating enzyme E1-like protein 2; E1-L2; MOP-4; UBE1L2; FLJ23367
• Gene ID: 55236
• mRNA Refseq: NM_018227
• Protein Refseq: NP_060697
• MIM: 611361
• UniProt ID: A0AVT1
• Chromosome Location: 4q13.2