Recombinant Mouse Uba6 Protein, Myc/DDK-tagged

• Cat.No.: Uba6-6769M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-like modifier activating enzyme 6 (Uba6), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 118 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Uba6 ubiquitin-like modifier activating enzyme 6 [ Mus musculus (house mouse) ]
• Official Symbol: Uba6
• Synonyms: UBA6; ubiquitin-like modifier activating enzyme 6; ubiquitin-like modifier-activating enzyme 6; ubiquitin-activating enzyme 6; ubiquitin-activating enzyme E1-like 2; ubiquitin-activating enzyme E1-like protein 2; E1-L2; Ube1l2; AU021846; AW124799; 4930542H01; 5730469D23Rik
• Gene ID: 231380
• mRNA Refseq: NM_172712
• Protein Refseq: NP_766300
• UniProt ID: Q8C7R4