Active Recombinant Human Tumor Protein P53

• Cat.No.: TP53-1076H

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: p53 wild type is His-tagged at the N-terminus. The protein was identified as a tumor suppressor by showing to block the transformation and to inhibit tumor cell growth. In addition, p53 is a transcription factor capable of regulating the expression of a subset of downstream genes. Different mutations in p53 gene have been characterized in a variety of human cancers. Loss or mutation of p53 function is highly correlated with tumorigenes
• Form: Liquid. Supplied in 20mM Tris-HCl pH8.0, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT.
• Activity: 1ng is sufficient for a gel mobility shift assay in a 20μl reaction, 50ng are sufficient for reconstituted transcription assay and 100ng are sufficient for a protein-protein interaction assay.
• Application: Recombinant p53 can be used for: (1) Mobility shift assay or for DNase I footprinting in the presence of double stranded DNA contain a consensus p53- binding sequence [5'-PuPuPuC (A/T) (T/A) GPyPyPy-3']. (2) In vitro transcription assa. (3) Rotein-protein interaction assay. (4) Cell growth assay.
• Purity: >95% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months. Store at -80℃. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: TP53 tumor protein p53 [ Homo sapiens ]
• Synonyms: TP53; tumor protein p53; p53; LFS1; TRP53; FLJ92943; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; p53 transformation suppressor; p53 tumor suppressor; transformation-related protein 53
• Gene ID: 7157
• mRNA Refseq: NM_000546
• Protein Refseq: NP_000537
• MIM: 191170
• UniProt ID: P04637
• Chromosome Location: 17p13.1
• Pathway: Amyotrophic lateral sclerosis (ALS); Apoptosis; Basal cell carcinoma; Bladder cancer; Cell cycle; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; Glioma; Huntington"s disease; MAPK signaling pathway; Melanoma; Neurotrophin signaling pathway; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Prostate cancer; Small cell lung cancer; Thyroid cancer; Wnt signaling pathway; p53 signaling pathway; Cell Cycle Checkpoints
• Function: ATP binding; DNA strand annealing activity; chaperone binding; chromatin binding; copper ion binding; enzyme binding; metal ion binding; promoter binding; protein N-terminus binding; protein heterodimerization activity; transcription factor activity; transcription factor binding; zinc ion binding