Active Recombinant Human TNFSF4 protein, Fc-tagged

• Cat.No.: TNFSF4-0589H
• Product Overview: Recombinant Human TNFSF4 protein(Gln51-Leu183), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 51-183 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human OX40 Protein, His Tag (MALS verified) at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 0.1-1 ng/mL. Immobilized Cynomolgus / Rhesus macaque OX40, His Tag at 2 μg/mL (100 μL/well) can bind Human OX40 Ligand, Fc Tag with a linear range of 0.2-3 ng/mL. Immobilized Biotinylated Human OX40, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Human OX40 Ligand, Fc Tag with a linear range of 0.1-0.4 ng/mL.
• Molecular Mass: Molecular CharacterizationThis protein carries a human IgG1 Fc tag at the N-terminus.The protein has a calculated MW of 74.0 kDa. The protein migrates as 130-210 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln51-Leu183
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TNFSF4 tumor necrosis factor (ligand) superfamily, member 4 [ Homo sapiens ]
• Official Symbol: TNFSF4
• Synonyms: TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tax transcriptionally activated glycoprotein 1, 34kD , TXGP1; tumor necrosis factor ligand superfamily member 4; CD252; gp34; OX 40L; OX40L; CD134 ligand; glycoprotein Gp34; OX40 antigen ligand; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); GP34; OX4OL; TXGP1; CD134L; OX-40L
• Gene ID: 7292
• mRNA Refseq: NM_003326
• Protein Refseq: NP_003317
• MIM: 603594
• UniProt ID: P23510