Active Recombinant Human TNFSF13, FLAG-tagged

• Cat.No.: TNFSF13-399H
• Product Overview: The extracellular domain of human APRIL (aa 98-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG-tag.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag
• Protein Length: 98-233;16-108 a.a.
• Description: APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. MegaAPRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.
• Form: Lyophilized. Contains PBS + 0.5% Trehalose.
• Bio-activity: Stimulates B cell proliferation.
• Molecular Mass: ~34kDa (SDS-PAGE)
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
• Concentration: 0.1mg/ml after reconstitution.

Gene Information

• Gene Name: TNFSF13 tumor necrosis factor (ligand) superfamily, member 13 [ Homo sapiens ]
• Official Symbol: TNFSF13
• Synonyms: TNFSF13; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; APRIL; CD256; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2; TALL2; ZTNF2; TALL-2; TRDL-1; FLJ57090; UNQ383/PRO715
• Gene ID: 8741
• mRNA Refseq: NM_001198622
• Protein Refseq: NP_001185551
• MIM: 604472
• UniProt ID: O75888
• Chromosome Location: 17p13.1
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Gene Expression, organism-specific biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem; Regulation of mRNA Stability by Proteins that Bind AU-rich Elements, organism-specific biosystem; Rheumatoid arthritis, organism-specific biosystem
• Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding