Active Recombinant Human TNF superfamily member 8 protein, His tagged

• Cat.No.: TNFSF8-1595H
• Product Overview: Active Recombinant Human TNF superfamily member 8 protein (Gln63-Asp234) with N-His tag was exprseed in Mouse myeloma cell line.

Specification

• Description: The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin''s and some non-Hodgkin''s lymphomas. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: N-His
• Protein length: 63-234 aa
• Bio-activity: Measured by its ability to stimulate IL-6 secretion by HDLM human Hodgkin''s lymphoma cells. The ED50 for this effect is 8-48 ng/mL in the presence of 10 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Molecular Mass: 22 kDa
• N-terminal Sequence Analysis: Met
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped at ambient temperature.
• References: 1. Brunangelo, F. et al. (1995) Blood 85:1.
  2. Duckett, C.S. et al. (1997) Mol. Cell. Biol. 17:1535.
  3. Chiarle, R. et al. (1999) J. Immunol. 163:194.

Gene Information

• Gene Name: TNFSF8 TNF superfamily member 8 [ Homo sapiens (human) ]
• Official Symbol: TNFSF8
• Synonyms: TNFSF8; tumor necrosis factor (ligand) superfamily, member 8; CD30LG; tumor necrosis factor ligand superfamily member 8; CD153; CD30-L; CD30 ligand; CD153 antigen; CD30 antigen ligand; CD30L; MGC138144
• Gene ID: 944
• mRNA Refseq: NM_001244
• Protein Refseq: NP_001235
• MIM: 603875
• UniProt ID: P32971