Active Recombinant Human TLR9 Full Length Transmembrane protein(Nanodisc)

• Cat.No.: TLR9-0183H
• Product Overview: Recombinant Human TLR9 Full Length Transmembrane protein(Q9NR96), was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Non
• Form: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Bio-activity: Elisa plates were pre-coated with Flag Tag TLR9-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TLR9-Nanodisc is 2.467ng/ml.
• Molecular Mass: The human full length TLR9 protein has a MW of 115.9 kDa.
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Different infant formulas can activate toll-like receptor 9 in vitro and inhibit interleukin 6 in human primary intestinal epithelial cells (2024)

Gene Information

• Gene Name: TLR9 toll-like receptor 9 [ Homo sapiens ]
• Official Symbol: TLR9
• Synonyms: TLR9; toll-like receptor 9; CD289
• Gene ID: 54106
• mRNA Refseq: NM_017442
• Protein Refseq: NP_059138
• MIM: 605474
• UniProt ID: Q9NR96

Reviews

02/27/2021

Fantastic results, very reliable.

08/08/2020

Great quality, excellent service.

01/24/2019

Satisfied with the purchase.

Q&As

In which cellular compartments is TLR9 primarily located?

TLR9 is primarily located in endosomes and lysosomes.

What is the primary role of TLR9 in the immune system?

TLR9 detects unmethylated CpG DNA from bacteria and viruses, activating immune responses.

Are there therapeutic strategies targeting TLR9?

Therapeutic strategies may target TLR9 to enhance vaccine efficacy or modulate autoimmune responses.

How is TLR9 implicated in autoimmune diseases?

Dysregulation of TLR9 can contribute to the development of autoimmune diseases.

How does TLR9 recognize and respond to foreign DNA?

It recognizes foreign DNA based on specific nucleotide patterns, initiating signaling cascades.

What types of pathogens does TLR9 help defend against?

It plays a role in defending against bacterial and viral infections.

What downstream signaling pathways does TLR9 activate?

TLR9 activation leads to NF-κB activation and cytokine production.