Active Recombinant Human THRA, His-tagged

• Cat.No.: THRA-1198H

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: His tagged TRα1 was expressed in a baculovirus system and purified by a combination of affinity and gel filtration chromatography. Nuclear receptors form the largest known family of transcription factors and have a crucial role in nearly all aspects of vertebrate development and adult physiology by transducing the effects of hormones into transcriptional responses. The family is defined by two domains: (a) the central, highly conserved, DNA-binding domain (DBD) of approx. 66 amino acids, and (b) the C-terminal, structurally conserved, ligand-binding domain (LBD) of approx. 250 amino acids. The aminoterminal regions are least conserved among nuclear receptor sequences. This domain is highly divergent between TRα and TRβ isoforms, which suggests differential roles in transcriptional regulation. In addition, alternative splicing of the TR-β gene generates two isoforms, TRα1 and TRβ2 with completely different amino-terminal domains. Unliganded TR inhibits the formation of a functional pre-initiation complex, through direct interaction with TBP and Transcription Factor IIB. In addition, in the absence of ligand TR has been shown to repress transcription through recruitment of a corepressor complex, which also includes Sin3A and histone deacetylase. Ligand binding releases the corepressor complex and recruits a coactivator complex that includes multiple histone acetyltransferases, including a steroid receptor family coactivator, p300/CREB-binding protein-associated factor (PCAF), and CREB binding protein (CBP).
• Form: Liquid. Supplied in 20 mM Tris-HCl pH 8.0, 100 mM KCl, 0.2 mM EDTA, 1 mM DTT and 20% glycerol.
• Purity: > 95% by SDS-PAGE.
• Application: TRα1 has been applied in in vitro transcription assays, DNA and protein-protein interactions assays.
• Activity: 20 ng are sufficient for an in vitro transcription assay and 100 ng are sufficient for a protein-protein interaction assay.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months store at -80°C. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: THRA thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian) [ Homo sapiens ]
• Synonyms: THRA; thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a) oncogene homolog, avian);AR7; EAR7; ERBA; ERBA1; NR1A1; THRA1; THRA2; ERB-T-1; MGC43240; c-ERBA-1; MGC000261; thyroid hormone receptor, alpha; ERBA-related 7; triiodothyronine receptor; thyroid hormone receptor alpha; avian erythroblastic leukemia viral (v-erb-a) oncogene homolog; EAR-7.1/EAR-7.2; THRA3; C-erbA-alpha; EAR-7; OTTHUMP00000164470; c-erbA-1; Nuclear receptor subfamily 1 group A member 1
• Gene ID: 7067
• mRNA Refseq: NM_003250
• Protein Refseq: NP_003241
• MIM: 190120
• UniProt ID: P10827
• Chromosome Location: 17q21.1
• Pathway: Neuroactive ligand-receptor interaction
• Function: protein binding; protein heterodimerization activity; protein homodimerization activity; single-stranded RNA binding; steroid hormone receptor activity; thyroid hormone receptor activity; transcription activator activity; transcription factor activity; transcription repressor activity; zinc ion binding; metal ion binding; promoter binding