Active Recombinant Human THBS2, His-tagged

• Cat.No.: THBS2-101H
• Product Overview: Recombinant Human THBS2 (Gly19­Ile1172) fused with a C­terminal and 10­His tag, was expressed in Mouse NSO Cells.

Specification

• Cat. No.: THBS2-101H
• Description: The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.
• Source: Mammalian cells
• Molecular Mass: Predicted molecular mass is 129 kDa. The apparent molecular mass of the protein is approximately 170­176 kDa in SDS-PAGE under reducing conditions.
• Predicted N terminal: Gly19
• Formulation: Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
• Purity: >90% as determined by SDS­PAGE
• Activity: Measured by the ability of the immobilized Protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. rhThrombospondin­2 is coated to 96 well plates at 10 µg/mL (100 µL/well) overnight then reduced with 20 mM DTT for 30 minutes. When 2 x 104 cells/well are added to rhThrombospondin­2 coated plates, approximately 55-75% will adhere after one hour at 37° C.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: THBS2

Gene Information

• Gene Name: THBS2 thrombospondin 2 [ Homo sapiens ]
• Synonyms: THBS2; thrombospondin 2; thrombospondin-2; TSP2
• Gene ID: 7058
• mRNA Refseq: NM_003247
• Protein Refseq: NP_003238
• MIM: 188061
• UniProt ID: P35442
• Chromosome Location: 6q27
• Pathway: ECM-receptor interaction; Focal Adhesion; Focal adhesion; Malaria
• Function: calcium ion binding; heparin binding; protein binding; structural molecule activity