Active Recombinant Human STING Protein, His tagged

• Cat.No.: STING-01H
• Product Overview: Recombinant N-terminal His-tagged STING catalytic domain (138-379 aa, R232 variant) purified from E. coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 138-379 aa
• Description: This gene encodes a five transmembrane protein that functions as a major regulator of the innate immune response to viral and bacterial infections. The encoded protein is a pattern recognition receptor that detects cytosolic nucleic acids and transmits signals that activate type I interferon responses. The encoded protein has also been shown to play a role in apoptotic signaling by associating with type II major histocompatibility complex. Mutations in this gene are the cause of infantile-onset STING-associated vasculopathy. Alternate splicing results in multiple transcript variants.
• Bio-activity: Serial dilutions of canonical 3'3'-cGAMP were incubated with 5 ug recombinant human STING R232 variant in 50 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol, and SYPRO Orange dye at 4 centigrade. The reaction was read on Real-Time PCR Detection System at 4-100 centigrade. The binding of the ligand stabilizes the protein structure. increasing the melting temperature (Tm), which is detected via a thermal shift assay (TSA), also known as a differential scanning fluorimetry(DSF) assay.
• Purity: ≥ 80% estimated by SDS-PAGE
• Stability: ≥ 2 years
• Storage: At -80 centigrade
• Storage Buffer: 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 10% glycerol
• Shipping: Dry ice in continental US; may vary elsewhere
• Reference: 1. Burdette, D.L., Monroe, K.M., Sotelo-Troha, K., et al. STING is a direct innate immune sensor of cyclic-di-GMP. Nature 478(7370), 515-518 (2011). 2. Sun, L., Wu, J., Du, F., et al. Cyclic GMP-AMP synthase is a cytosolic DNA sensor that activates the type I interferon pathway. Science 339(6121), 786-791 (2013). 3. Wu, J., Sun, L., Chen, X., et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121), 826-830 (2013).

Gene Information

• Gene Name: STING1 stimulator of interferon response cGAMP interactor 1 [ Homo sapiens (human) ]
• Official Symbol: STING1
• Synonyms: STING1; stimulator of interferon response cGAMP interactor 1; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; TMEM173; STING-beta; stimulator of interferon genes protein; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; endoplasmic reticulum IFN stimulator; endoplasmic reticulum interferon stimulator; mitochondrial mediator of IRF3 activation; stimulator of interferon protein; stimulator of interferon response cGAMP interactor-deltaC; stimulator of interferon response cGAMP interactor-deltaN; sting 1; transmembrane protein 173
• Gene ID: 340061
• mRNA Refseq: NM_198282
• Protein Refseq: NP_938023
• MIM: 612374
• UniProt ID: Q86WV6