Active Recombinant Human SPP1 Protein

• Cat.No.: SPP1-705H
• Product Overview: Recombinant Human SPP1, transcript variant 2, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293 cells
• Species: Human
• Form: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer, 100mM NaCl, pH 7.2
• Bio-activity: Determined by its ability to chemoattract human monocytes using a concentration range of 10.0-100.0 ng/ml.
• Molecular Mass: 60 kDa
• AA Sequence: IPVKQADSGSSEEKQLYNKYPDAVA TWLNPDPSQKQNLLAPQNAVSSEET NDFKQETLPSKSNESHDHMDDMDDE DDDDHVDSQDSIDSNDSDDVDDTDD SHQSDESHHSDESDELVTDFPTDLP ATEVFTPVVPTVDTYDGRGDSVVYG LRSKSKKFRRPDIQYPDATDEDITS HMESEELNGAYKAIPVAQDLNAPSD WDSRGKDSYETSQLDDQSAETHSHK QSRLYKRKANDESNEHSDVIDSQEL SKVSREFHSHEFHSHEDMLVVDPKS KEEDKHLKFRISHELDSASSEVN
• Endotoxin: Endotoxin level is <0.1 ng/µg of protein (<1EU/µg).
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: Resuspend the protein in the desired concentration in proper buffer
• Tag: Non

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens ]
• Official Symbol: SPP1
• Synonyms: SPP1; secreted phosphoprotein 1; BNSP, bone sialoprotein I , OPN, osteopontin; osteopontin; BSPI; early T lymphocyte activation 1; ETA 1; uropontin; nephropontin; osteopontin-C; osteopontin-D; SPP1/CALPHA1 fusion; bone sialoprotein 1; urinary stone protein; early T-lymphocyte activation 1; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); OPN; BNSP; ETA-1; MGC110940
• Gene ID: 6696
• mRNA Refseq: NM_000582
• Protein Refseq: NP_000573
• MIM: 166490
• UniProt ID: P10451