Recombinant Human Secreted Phosphoprotein 1, His-tagged

• Cat.No.: SPP1-372H
• Product Overview: Recombinant human Osteopontin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography, 36.2 kDa (321aa).

Specification

• Cat. No.: SPP1-372H
• Description: Osteopontin (OPN), also known as Secreted phosphoprotein 1 (SPP1), is a human gene product which is conserved in other species. This protein is glycoprotein that was first identified in osteoblasts and plays an important role in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH RSMIPVKQAD SGSSEEKQLY NKYPDAVATW LNPDPSQKQN LLAPQNAVSS EETNDFKQET LPSKSNESHD HMDDMDDEDD DDHVDSQDSI DSNDSDDVDD TDDSHQSDES HHSDESDELV TDFPTDLPAT EVFTPVVPTV DTYDGRGDSV VYGLRSKSKK FRRPDIQYPD ATDEDITSHM ESEELNGAYK AIPVAQDLNA PSDWDSRGKD SYETSQLDDQ SAETHSHKQS RLYKRKANDE SNEHSDVIDS QELSKVSREF HSHEFHSHED MLVVDPKSKE EDKHLKFRIS HELDSASSEV N
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% glycerol, 2 mM EDTA
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SPP1 secreted phosphoprotein 1 [ Homo sapiens ]
• Synonyms: SPP1; secreted phosphoprotein 1; OPN; BNSP; BSPI; ETA-1; MGC110940; SPP1/CALPHA1 fusion; bone sialoprotein I; early T-lymphocyte activation 1; secreted phosphoprotein-1 (osteopontin, bone sialoprotein); osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; Urinary stone protein; Nephropontin; Uropontin; PSEC0156; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
• Gene ID: 6696
• mRNA Refseq: NM_ 000582
• Protein Refseq: NP_ 000573
• MIM: 166490
• UniProt ID: P10451
• Chromosome Location: 4q21-q25
• Pathway: Cell Communication; ECM-receptor interaction; Focal adhesion; Integrin cell surface interactions
• Function: cytokine activity; protein binding