Recombinant Rat Sost protein(Met1-Tyr213), His-tagged

• Cat.No.: Sost-3326R
• Product Overview: Recombinant Rat SOST (NP_085073.1) (Met 1-Tyr 213) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 1-213 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10 T1/2 cells. The ED50 for this effect is typically 0.1-0.5 μg/mL.
• Molecular Mass: The recombinant rat SOST comprises 201 amino acids and predicts a molecular mass of 23 kDa. The apparent molecular mass of the rat SOST is approximately 33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Sost sclerostin [ Rattus norvegicus ]
• Official Symbol: Sost
• Synonyms: SOST; sclerostin; sclerosteosis
• Gene ID: 80722
• mRNA Refseq: NM_030584
• Protein Refseq: NP_085073