Active Recombinant Human SOST protein, His-tagged

• Cat.No.: SOST-870H
• Product Overview: Recombinant Human SOST (NP_079513.1) (Gln24-Tyr213) was expressed in HEK293, fused with a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Gln24-Tyr213
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit Wnt­3a­ induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is typically 1.5-6μg/mL in the presence of 3ng/mL Recombinant Mouse Wnt­3a. Measured by its ability to inhibit BMP­6 ­induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. 30µg/mL of rhSOST will antagonize hBMP­6 induction of alkaline phosphatase in MC3T3E1 cells by 50­80%.
• Molecular Mass: The recombinant human SOST is a monomeric protein consists of 197 amino acids and predicts a molecular mass of 22.5 kDa. As a result of glycosylation, the apparent molecular mass of rhSOST is approximately 29.9 kDa in SDS-PAGE under reducing conditions..
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SOST sclerostin [ Homo sapiens ]
• Official Symbol: SOST
• Synonyms: SOST; sclerostin; sclerosteosis; VBCH
• Gene ID: 50964
• mRNA Refseq: NM_025237
• Protein Refseq: NP_079513
• MIM: 605740
• UniProt ID: Q9BQB4